Human Type IV Collagen ELISA Kit (HDES0091)
- SKU:
- HDES0091
- Size:
- 96T
- Detection Method:
- Sandwich ELISA
- Target:
- cIV
- Detection Wavelength:
- 450/630nm
Description
| Product Name: | Human Type IV Collagen ELISA Kit |
| SKU: | HDES0091 |
| Size: | 96T |
| Target: | cIV |
| Detection Method: | Sandwich ELISA |
| Result Type: | Quantitative: Sensitivity 98.4%, Specificity 99.9% |
| Sample Type: | Serum |
| Assay Time: | 1 h 45 min |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Sandwich-ELISA as the method to detect the Type IV Collagen (cIV) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with cIV monoclonal antibody. Samples and cIV polyclonal antibody are sequentially added to the ELISA Microtiter plate wells, after wash, add HRP Conjugate. If cIV present in the sample, it will combine with the pre-coated cIV monoclonal antibody to form antibody-antigen-antibody- HRP Conjugate compound. Wash the Microtiter plate to remove unbound HRP Conjugates. The substrate reagent is added to initiate the color developing reaction. The presence of cIV can be determined by measuring the absorbance value by using a microplate reader with 450 nm wavelength. The OD value is proportional to the concentration of cIV. You can calculate the concentration of cIV in the samples by comparing the OD of the samples to the standard curve.
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