Human TPMT Recombinant Protein (RPPB2670)
- SKU:
- RPPB2670
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- TPMT
- Synonyms:
- TPMT
- Thiopurine S-methyltransferase
- EC 21167
- Thiopurine methyltransferase
- Source:
- Escherichia Coli
- Uniprot:
- P51580
Description
| Product Name: | Human TPMT Recombinant Protein |
| Product Code: | RPPB2670 |
| Size: | 20µg |
| Species: | Human |
| Target: | TPMT |
| Synonyms: | TPMT, Thiopurine S-methyltransferase, EC 2.1.1.67, Thiopurine methyltransferase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 1 mg/ml solution containing 20mM Tris 8.0, 0.2mM PMSF and 2mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGKSGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKVFKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLYLLTEK |
TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.
TPMT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 245 amino acids (1-245) and having a molecular mass of 28 kDa. Thiopurine S-methyltransferase is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.1.1.67; Methyltransferase Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: cytosol Molecular Function:thiopurine S-methyltransferase activity Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Disease: Thiopurine S-methyltransferase Deficiency |
| NCBI Summary: | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014] |
| UniProt Code: | P51580 |
| NCBI GenInfo Identifier: | 1730006 |
| NCBI Gene ID: | 7172 |
| NCBI Accession: | P51580.1 |
| UniProt Secondary Accession: | P51580,O14806, O15423, O15424, O15425, O15426, O15515 O15548, O43213, Q5VUK6, Q9UBE6, Q9UBT8, |
| UniProt Related Accession: | P51580 |
| Molecular Weight: | 245 |
| NCBI Full Name: | Thiopurine S-methyltransferase |
| NCBI Synonym Full Names: | thiopurine S-methyltransferase |
| NCBI Official Symbol: | TPMT |
| NCBI Protein Information: | thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase |
| UniProt Protein Name: | Thiopurine S-methyltransferase |
| UniProt Synonym Protein Names: | Thiopurine methyltransferase |
| UniProt Gene Name: | TPMT |
| UniProt Entry Name: | TPMT_HUMAN |
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