The Human Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) ELISA Kit is a specialized assay designed to quantitatively measure levels of sVEGFR-1 in various human biological samples.
sVEGFR-1 plays a crucial role in regulating angiogenesis by sequestering vascular endothelial growth factor (VEGF), thereby influencing vascular development, endothelial cell function, and potentially impacting processes like tumor growth and neovascular diseases. Our sVEGFR-1 ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactured under strict quality control standards, this kit provides robust performance and user-friendly protocols, making it an ideal choice for investigating sVEGFR-1 levels and their implications in physiological and pathological conditions. Trust Assay Genie for precise quantification and in-depth analysis of sVEGFR-1 biomarkers in your studies.
Human sVEGFR-1 (soluble Vascular endothelial growth factor receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sVEGFR-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sVEGFR-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sVEGFR-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sVEGFR-1. You can calculate the concentration of Human sVEGFR-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
96-103
88-98
Average (%)
101
99
93
1:4
Range (%)
97-111
90-106
86-98
Average (%)
104
98
91
1:8
Range (%)
88-102
90-105
87-94
Average (%)
96
97
90
1:16
Range (%)
96-108
90-98
97-109
Average (%)
102
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
104
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
89-101
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.72
51.6
224.67
22.05
58.45
237.57
Standard deviation
1.16
2.53
10.31
1.28
2.76
12.54
C V (%)
5.34
4.9
4.59
5.8
4.72
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sVEGFR-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sVEGFR-1 in samples. No significant cross-reactivity or interference between Human sVEGFR-1 and analogues was observed.
