Human RPS27A Recombinant Protein (RPPB5064)
- SKU:
- RPPB5064
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- RPS27A
- Synonyms:
- Ubiquitin
- Ribosomal Protein S27a
- CEP80
- UBA80
- Source:
- Escherichia Coli
- Uniprot:
- P62988
Description
| Product Name: | Human RPS27A Recombinant Protein |
| Product Code: | RPPB5064 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPS27A |
| Synonyms: | Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless liquid formulation. |
| Formulation: | The RPS27A is supplied in 1x PBS and 0.05% PBS. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | RPS27A Protein biotinilation is determined by Western Blotting and ELISA analysis using streptavidin–HRP conjugated as a detection reagent. Free biotin is eliminated by dialysis against PBS. Protein concentration is determined by 280nm absorbance. |
Recombinant Human Ubiquitin having the accession number of P62988 was conjugated to Biotin. RPS27A is a small protein composed of 76 amino acids. RPS27A is found only in eukaryotic organisms among which shows strong sequence conservation. The RPS27A protein is present in all cell types, thus giving rise to its name.RPS27A is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end and the side chains of lysine.The connection of multiple copies of RPS27A targets the proteins for degradation by the 26S proteosome. RPS27A ligation is an ATP-dependent multi-step process. RPS27A is activated by the E1 enzyme. The attachment of RPS27A to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which is involved in the recognition of the substrate protein.
Recombinant Human RPS27A protein biotinylated with NHS-biotin produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 76 amino acids and having a molecular mass of 8.6 kDa.
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62988 |
| NCBI GenInfo Identifier: | 51703339 |
| NCBI Gene ID: | 7311 |
| NCBI Accession: | P62988.1 |
| UniProt Secondary Accession: | P62988 |
| UniProt Related Accession: | P62988,P62987 |
| Molecular Weight: | |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | ubiquitin A-52 residue ribosomal protein fusion product 1 |
| NCBI Official Symbol: | UBA52 |
| NCBI Official Synonym Symbols: | L40; CEP52; RPL40; HUBCEP52 |
| NCBI Protein Information: | ubiquitin-60S ribosomal protein L40 |
| UniProt Protein Name: | |
| UniProt Synonym Protein Names: | |
| Protein Family: | Ubiquitin |
| UniProt Gene Name: | |
| UniProt Entry Name: |
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