Human PTTG1 Recombinant Protein (RPPB4334)
- SKU:
- RPPB4334
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PTTG1
- Synonyms:
- Securin
- Pituitary tumor-transforming gene 1 protein
- hPTTG
- Tumor-transforming protein 1
- Source:
- Escherichia Coli
- Uniprot:
- O95997
Description
| Product Name: | Human PTTG1 Recombinant Protein |
| Product Code: | RPPB4334 |
| Size: | 20µg |
| Species: | Human |
| Target: | PTTG1 |
| Synonyms: | Securin, Pituitary tumor-transforming gene 1 protein, hPTTG, Tumor-transforming protein 1, Esp1-associated protein, PTTG1, EAP1, PTTG, TUTR1, MGC126883, MGC138276. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PTTG1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 20% glycerol, 1mM EDTA and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI |
PTTG1 (Securin) is mainly involved in the regulation of sister chromatid separation during cell division. Securin is a regulatory protein, which has a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Additional two roles have been identified for PTTG1; the first one is to facilitate the transport of separase (cysteine protease) to the nucleus and the second role is to hinder the catalytic activity of separase. PTTG1 is ubiquitinated by the APC (Anaphase Promoting Complex), and subsequently degraded by the Proteasome, releasing separase. During the mitosis, PTTG1 blocks Separase/ESPL1 function, thus preventing the proteolysis of the cohesin complex and the ensuing segregation of the chromosomes. However, PTTG1 function is not restricted to a blocking activity only, since it is required to activate ESPL1. PTTG1 is ubiquitinated at the beginning of anaphase, leading to its destruction and to the liberation of ESPL1. PTTG1 contains two PXXP motifs, which are necessary for its transforming and tumorigenic activities, in addition to its stimulation of basic fibroblast growth factor expression. It also contains a D box (destruction box) which is essential for its degradation by the APC. The acidic C-terminal region of the Securin can function as a transactivation domain. Even though, PTTG1 is primarily a cytosolic protein, it partially localizes in the nucleus.PTTG1 is highly expressed in various tumors; it also has transforming activity in vitro and tumorigenic activity in vivo. Furthermore, PTTG1 negatively regulates the transcriptional activity and related apoptosis activity of TP53.
PTTG1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (1- 202 a.a.) and having a molecular mass of 24.1kDa.The PTTG1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Securin: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. Belongs to the securin family. |
| UniProt Protein Details: | Protein type:Oncoprotein; Cell cycle regulation Chromosomal Location of Human Ortholog: 5q35.1 Cellular Component: cytoplasm; cytosol Molecular Function:protease inhibitor activity; protein binding; transcription factor activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; homologous chromosome segregation; protein ubiquitination during ubiquitin-dependent protein catabolic process; spermatogenesis; transcription from RNA polymerase II promoter |
| NCBI Summary: | The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Code: | O95997 |
| NCBI GenInfo Identifier: | 29336594 |
| NCBI Gene ID: | 9232 |
| NCBI Accession: | O95997.1 |
| UniProt Related Accession: | O95997 |
| Molecular Weight: | |
| NCBI Full Name: | Securin |
| NCBI Synonym Full Names: | pituitary tumor-transforming 1 |
| NCBI Official Symbol: | PTTG1 |
| NCBI Official Synonym Symbols: | EAP1; PTTG; HPTTG; TUTR1 |
| NCBI Protein Information: | securin |
| UniProt Protein Name: | Securin |
| UniProt Synonym Protein Names: | Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG |
| UniProt Gene Name: | PTTG1 |
| UniProt Entry Name: | PTTG1_HUMAN |
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