Human PSME3 Recombinant Protein (RPPB4325)
- SKU:
- RPPB4325
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- PSME3
- Synonyms:
- Proteasome (prosome
- macropain) activator subunit 3 (PA28 gamma
- Ki)
- PA28G
- Source:
- Escherichia Coli
- Uniprot:
- P61289
Description
| Product Name: | Human PSME3 Recombinant Protein |
| Product Code: | RPPB4325 |
| Size: | 10µg |
| Species: | Human |
| Target: | PSME3 |
| Synonyms: | Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki), PA28G, Ki, Ki nuclear autoantigen, PA28-gamma, REG-GAMMA, Activator of multicatalytic protease subunit 3, Proteasome activator 28 subunit gamma, 11S regulator complex subunit gamma, PA28gamma, |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PSME3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 2mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY |
PSME3 is a member of the PA28 family. The 26S proteasome is an extremely organized multicatalytic proteinase complex composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are spread all over the eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 stimulates the trypsin-like catalytic subunit of the proteasome and inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 enables the MDM2-p53/TP53 collaboration that encourages ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, restricting its growth and causing inhibited apoptosis after DNA damage.
PSME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 274 amino acids (1-254 a.a.) and having a molecular mass of 31.7kDa. PSME3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; Nuclear receptor co-regulator; Proteasome complex Chromosomal Location of Human Ortholog: 17q21 Cellular Component: proteasome complex; nucleoplasm; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein binding; p53 binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012] |
| UniProt Code: | P61289 |
| NCBI GenInfo Identifier: | 47117724 |
| NCBI Gene ID: | 10197 |
| NCBI Accession: | P61289.1 |
| UniProt Secondary Accession: | P61289,O35563, P97373, Q12920, Q13172, Q9BQD9, A8K9A3 |
| UniProt Related Accession: | P61289 |
| Molecular Weight: | 254 |
| NCBI Full Name: | Proteasome activator complex subunit 3 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) |
| NCBI Official Symbol: | PSME3 |
| NCBI Official Synonym Symbols: | Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma |
| NCBI Protein Information: | proteasome activator complex subunit 3; Ki antigen; REG gamma-3; PA28 gamma variant 5; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; epididymis secretory protein Li 283; proteasome activator 28 subunit gamma; activator of multicatalytic protease subunit 3 |
| UniProt Protein Name: | Proteasome activator complex subunit 3 |
| UniProt Synonym Protein Names: | 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma |
| Protein Family: | Proteasome activator |
| UniProt Gene Name: | PSME3 |
| UniProt Entry Name: | PSME3_HUMAN |
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