Description
| Product Name: | Human PPP3R1 Recombinant Protein |
| Product Code: | RPPB2171 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPP3R1 |
| Synonyms: | Protein phosphatase 3 regulatory subunit B alpha, CNB1, CALNB1, Protein phosphatase 3 (formerly 2B) regulatory subunit B (19kD) alpha isoform (calcineurin B type I), Calcineurin B type I (19kDa). |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | PPP3R1 Human solution (1mg/1ml) containing 20mM Trsi HCL pH-8, 0.1M NaCl, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV |
PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
PPP3R1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing�190 amino acids (1-170.a.a) and having a molecular mass of 21.5kDa. PPP3R1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family. |
| UniProt Protein Details: | Protein type:Protein phosphatase, regulatory subunit; Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p15 Cellular Component: nucleoplasm; cytosol; calcineurin complex; sarcolemma Molecular Function:calmodulin binding; protein domain specific binding; protein binding; calcium-dependent protein serine/threonine phosphatase activity; calcium ion binding Biological Process: calcineurin-NFAT signaling pathway; positive regulation of NFAT protein import into nucleus; dephosphorylation; apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | P63098 |
| NCBI GenInfo Identifier: | 52000904 |
| NCBI Gene ID: | 5534 |
| NCBI Accession: | P63098.2 |
| UniProt Related Accession: | P63098 |
| Molecular Weight: | |
| NCBI Full Name: | Calcineurin subunit B type 1 |
| NCBI Synonym Full Names: | protein phosphatase 3 regulatory subunit B, alpha |
| NCBI Official Symbol: | PPP3R1�� |
| NCBI Official Synonym Symbols: | CNB; CNB1; CALNB1�� |
| NCBI Protein Information: | calcineurin subunit B type 1 |
| UniProt Protein Name: | Calcineurin subunit B type 1 |
| UniProt Synonym Protein Names: | Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1 |
| UniProt Gene Name: | PPP3R1�� |
| UniProt Entry Name: | CANB1_HUMAN |
