Human PPCS Recombinant Protein (RPPB2152)
- SKU:
- RPPB2152
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PPCS
- Synonyms:
- Phosphopantothenate--cysteine ligase
- Phosphopantothenoylcysteine synthetase
- PPC synthetase
- PPCS
- Source:
- Escherichia Coli
- Uniprot:
- Q9HAB8
Description
| Product Name: | Human PPCS Recombinant Protein |
| Product Code: | RPPB2152 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPCS |
| Synonyms: | Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB, RP11-163G10.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PPCS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol. |
| Stability: | PPCS Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N |
Phosphopantothenate-cysteine ligase (PPCS) catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is a vital universal pathway in prokaryotes and eukaryotes. PPCS, being one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
PPCS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 331 amino acids (1-311 a.a.) and having a molecular mass of 36.1kDa.PPCS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPCS: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. Belongs to the PPC synthetase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 6.3.2.5; Ligase Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytosol Molecular Function:phosphopantothenate--cysteine ligase activity Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process |
| NCBI Summary: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9HAB8 |
| NCBI GenInfo Identifier: | 47117318 |
| NCBI Gene ID: | 79717 |
| NCBI Accession: | Q9HAB8.2 |
| UniProt Secondary Accession: | Q9HAB8,Q3KQT2, Q5VVM0, |
| UniProt Related Accession: | Q9HAB8 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | Phosphopantothenate--cysteine ligase |
| NCBI Synonym Full Names: | phosphopantothenoylcysteine synthetase |
| NCBI Official Symbol: | PPCS |
| NCBI Protein Information: | phosphopantothenate--cysteine ligase |
| UniProt Protein Name: | Phosphopantothenate--cysteine ligase |
| UniProt Synonym Protein Names: | Phosphopantothenoylcysteine synthetase; PPC synthetase |
| Protein Family: | Phosphopantothenate--cysteine ligase |
| UniProt Gene Name: | PPCS |
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