Human PPCDC Recombinant Protein (RPPB2151)
- SKU:
- RPPB2151
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- PPCDC
- Synonyms:
- FLJ14585
- PPC-DC
- MDS018
- COAC
- Source:
- Escherichia Coli
- Uniprot:
- Q96CD2
Description
| Product Name: | Human PPCDC Recombinant Protein |
| Product Code: | RPPB2151 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPCDC |
| Synonyms: | FLJ14585, PPC-DC, MDS018, COAC, CoaC, Phosphopantothenoylcysteine Decarboxylase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PPCDC protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer(pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS |
PPCDC is a member of the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily which takes a part in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate needs several steps: the phosphorylation of pantothenate, the conversion of 4’-hosphopantothenate to 4''-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC is a key player in this pathway. PPCDC converts 4''-phosphopantothenate into 4''-phosphopantetheine.
PPCDC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204a.a.) and having a molecular mass of 24.6kDa.PPCDC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPCDC: Necessary for the biosynthesis of coenzyme A. Catalyzes the decarboxylation of 4-phosphopantothenoylcysteine to form 4'- phosphopantotheine. Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 4.1.1.36; Lyase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesisChromosomal Location of Human Ortholog: 15q24.2Cellular Component: cytosolMolecular Function: phosphopantothenoylcysteine decarboxylase activity; protein bindingBiological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96CD2 |
| NCBI GenInfo Identifier: | 296434457 |
| NCBI Gene ID: | 60490 |
| NCBI Accession: | Q96CD2.2 |
| UniProt Secondary Accession: | Q96CD2,Q96SX0, Q9HC17 |
| UniProt Related Accession: | Q96CD2 |
| Molecular Weight: | 14,069 Da |
| NCBI Full Name: | Phosphopantothenoylcysteine decarboxylase |
| NCBI Synonym Full Names: | phosphopantothenoylcysteine decarboxylase |
| NCBI Official Symbol: | PPCDC |
| NCBI Official Synonym Symbols: | coaC; MDS018; PPC-DC |
| NCBI Protein Information: | phosphopantothenoylcysteine decarboxylase |
| UniProt Protein Name: | Phosphopantothenoylcysteine decarboxylase |
| UniProt Synonym Protein Names: | CoaC |
| Protein Family: | Putative phosphopantothenoylcysteine decarboxylase |
| UniProt Gene Name: | PPCDC |
| UniProt Entry Name: | COAC_HUMAN |
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