Human POLR2E Recombinant Protein (RPPB2137)
- SKU:
- RPPB2137
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- POLR2E
- Synonyms:
- DNA-directed RNA polymerases I
- II
- and III subunit RPABC1
- RNA polymerases I
- Source:
- Escherichia Coli
- Uniprot:
- P19388
Description
| Product Name: | Human POLR2E Recombinant Protein |
| Product Code: | RPPB2137 |
| Size: | 20µg |
| Species: | Human |
| Target: | POLR2E |
| Synonyms: | DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E, RPB5, RPABC1, hRPB25, hsRPB5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The POLR2E solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVTELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ |
DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) is a member of the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E being a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. POLR2E is the 5th largest subunit of RNA polymerase II (the polymerase which is responsible for synthesizing messenger RNA in eukaryotes). The POLR2E subunit is shared by the other 2 DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between the POLR2E subunit and a hepatitis virus transactivating protein has been established, suggesting that an interaction between transcriptional activators and the polymerase can occur through the POLR2E subunit.
POLR2E Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210) and having a molecular mass of 27.1kDa.POLR2E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex Molecular Function:protein binding; DNA binding; DNA-directed RNA polymerase activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of gene expression, epigenetic; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase III promoter; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter |
| NCBI Summary: | This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19388 |
| NCBI GenInfo Identifier: | 116242767 |
| NCBI Gene ID: | 5434 |
| NCBI Accession: | P19388.4 |
| UniProt Secondary Accession: | P19388,O43380, Q6PIH5, Q9BT06, B2R6L4, D6W5Y1, |
| UniProt Related Accession: | P19388 |
| Molecular Weight: | 210 |
| NCBI Full Name: | DNA-directed RNA polymerases I, II, and III subunit RPABC1 |
| NCBI Synonym Full Names: | polymerase (RNA) II (DNA directed) polypeptide E, 25kDa |
| NCBI Official Symbol: | POLR2E |
| NCBI Official Synonym Symbols: | RPB5; XAP4; RPABC1; hRPB25; hsRPB5 |
| NCBI Protein Information: | DNA-directed RNA polymerases I, II, and III subunit RPABC1; RPB5 homolog; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerase subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide |
| UniProt Protein Name: | DNA-directed RNA polymerases I, II, and III subunit RPABC1 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4 |
| UniProt Gene Name: | POLR2E |
| UniProt Entry Name: | RPAB1_HUMAN |
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