Human POFUT1 Recombinant Protein (RPPB2132)
- SKU:
- RPPB2132
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- POFUT1
- Synonyms:
- FUT12
- O-Fuc-T
- O-FucT-1
- O-FUT
- Source:
- Escherichia Coli
- Uniprot:
- Q9H488
Description
| Product Name: | Human POFUT1 Recombinant Protein |
| Product Code: | RPPB2132 |
| Size: | 20µg |
| Species: | Human |
| Target: | POFUT1 |
| Synonyms: | FUT12, O-Fuc-T, O-FucT-1, O-FUT, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltraferase 1,KIAA0180. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The POFUT1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSGSWDPAG YLLYCPCMGR FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL EPLQAYHRVI SLEDFMEKLA PTHWPPEKRV AYCFEVAAQR SPDKKTCPMK EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YREQWSQRFS PKEHPVLALP GAPAQFPVLE EHRPLQKYMV WSDEMVKTGE AQIHAHLVRP YVGIHLRIGS DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TAAPLTMTMC LPDLKEIQRA VKLWVRSLDA QSVYVATDSE SYVPELQQLF KGKVKVVSLK PEVAQVDLYI LGQADHFIGN CVSSFTAFVK RERDLQGRPS SFFGMDRPPK LRDEF |
GDP-fucose protein O-fucosyltransferase 1 (POUFUT1), belongs to the glycosyltransferase O-Fuc family. POUFUT1 encodes a member of the glycosyltransferase O-Fuc family and Expressed mainly in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle.POUFUT1 adds O-fucose through an O-glycosidic linkage to Preserve serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and emitted proteins. POUFUT1 is involved in ligand-induced receptor signaling. Alternative splicing of this gene results in 2 transcript variants encoding different isoforms.POFUT1 participates in Notch signaling, as Notch ligands can use as POFUT1 substrates.
POFUT1 Human Recombinant produced in E. coli is a single polypeptide chain containing 385 amino acids (27-388) and having a molecular mass of 43.7 kDa. POFUT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POFUT1: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. Belongs to the glycosyltransferase 68 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.4.1.221 Chromosomal Location of Human Ortholog: 20q11 Cellular Component: endoplasmic reticulum; membrane Molecular Function:fucosyltransferase activity; peptide-O-fucosyltransferase activity Biological Process: O-glycan processing; protein amino acid O-linked glycosylation Disease: Dowling-degos Disease 2 |
| NCBI Summary: | This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H488 |
| NCBI GenInfo Identifier: | 23396787 |
| NCBI Gene ID: | 23509 |
| NCBI Accession: | Q9H488.1 |
| UniProt Secondary Accession: | Q9H488,Q14685, Q5W185, Q9BW76, A8K4R8, E1P5M4, |
| UniProt Related Accession: | Q9H488 |
| Molecular Weight: | 22,338 Da |
| NCBI Full Name: | GDP-fucose protein O-fucosyltransferase 1 |
| NCBI Synonym Full Names: | protein O-fucosyltransferase 1 |
| NCBI Official Symbol: | POFUT1 |
| NCBI Official Synonym Symbols: | DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1 |
| NCBI Protein Information: | GDP-fucose protein O-fucosyltransferase 1 |
| UniProt Protein Name: | GDP-fucose protein O-fucosyltransferase 1 |
| UniProt Synonym Protein Names: | Peptide-O-fucosyltransferase 1; O-FucT-1 |
| UniProt Gene Name: | POFUT1 |
| UniProt Entry Name: | OFUT1_HUMAN |
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