Human PNMT Recombinant Protein (RPPB2129)
- SKU:
- RPPB2129
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- PNMT
- Synonyms:
- PENT
- PNMTase
- Noradrenaline-N-methyltransferase
- Phenylethanolamine N-methyltransferase
- Source:
- Escherichia Coli
- Uniprot:
- P11086
Description
| Product Name: | Human PNMT Recombinant Protein |
| Product Code: | RPPB2129 |
| Size: | 25µg |
| Species: | Human |
| Target: | PNMT |
| Synonyms: | PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase, PNMT, MGC34570. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PNMT protein solution contains 20mM Tris-HCl, pH-8 & 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL |
PNMT is an enzyme located in the adrenal medulla and it catalyzes the final step of the catecholamine biosynthesis pathway. PNMT has beta-carboline 2N-methyltransferase activity. PNMT takes part in regulating epinephrine production. Glucocorticoid receptors form multimers of PNMT independent of the DNA binding domain. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids.
Recombinant Human PNMT produced in E.Coli is a single, non-glycosylated polypeptide chain containing 282 amino acids (1-282 a.a.) and having a molecular mass of 30.8 kDa.PNMT is purified by conventional chromatographic techniques.
| UniProt Protein Function: | PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.28; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cytosol Molecular Function:phenylethanolamine N-methyltransferase activity Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process Disease: Hypertension, Essential |
| NCBI Summary: | The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012] |
| UniProt Code: | P11086 |
| NCBI GenInfo Identifier: | 130375 |
| NCBI Gene ID: | 5409 |
| NCBI Accession: | P11086.1 |
| UniProt Related Accession: | P11086 |
| Molecular Weight: | 282 |
| NCBI Full Name: | Phenylethanolamine N-methyltransferase |
| NCBI Synonym Full Names: | phenylethanolamine N-methyltransferase |
| NCBI Official Symbol: | PNMT |
| NCBI Official Synonym Symbols: | PENT; PNMTase |
| NCBI Protein Information: | phenylethanolamine N-methyltransferase; phenylethanolamine N-methylase; noradrenaline N-methyltransferase |
| UniProt Protein Name: | Phenylethanolamine N-methyltransferase |
| UniProt Synonym Protein Names: | Noradrenaline N-methyltransferase |
| Protein Family: | Phenylethanolamine N-methyltransferase |
| UniProt Gene Name: | PNMT |
| UniProt Entry Name: | PNMT_HUMAN |
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