Description
| Product Name: | Human PLA2G12 Recombinant Protein |
| Product Code: | RPPB2119 |
| Size: | 10µg |
| Species: | Human |
| Target: | PLA2G12 |
| Synonyms: | Group XIIA secretory phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase GXII, GXII sPLA2, PLA2G12, sPLA2-XII, PLA2G12A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4�m) and lyophilized from 0.5 mg/ml in 0.01M Tris buffer pH 8.6. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95% as determined by SDS PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASHMQEQA QTTDWRATLK TIRNGVHKID TYLNAALDLL�GGEDGLCQYK CSDGSKPFPR YGYKPSPPNG CGSPLFGVHLNIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLTQ HVQACETTVE LLFDSVIHLG CKPYLDSQRAACRCHYEEKT DL |
Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.
Secreted Phospholipase A2-XII Human Recombiannt was produced with N-terminal His-Tag. PLA2G12 His-Tagged Fusion Protein is 20.6 kDa containing 167 amino acid residues of the human secreted phospholipase A2-XII and 16 additional amino acid residues � His-Tag (underlined).
| UniProt Protein Function: | PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; Secreted; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - linoleic acid; Secreted, signal peptide; EC 3.1.1.4; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid; Endoplasmic reticulum; Phospholipase Chromosomal Location of Human Ortholog: 4q25 Cellular Component: extracellular region Molecular Function:phospholipase A2 activity Biological Process: phosphatidic acid biosynthetic process |
| NCBI Summary: | Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BZM1 |
| NCBI GenInfo Identifier: | 20143881 |
| NCBI Gene ID: | 81579 |
| NCBI Accession: | Q9BZM1.1 |
| UniProt Secondary Accession: | Q9BZM1,Q9BZ89, |
| UniProt Related Accession: | Q9BZM1 |
| Molecular Weight: | 21,067 Da |
| NCBI Full Name: | Group XIIA secretory phospholipase A2 |
| NCBI Synonym Full Names: | phospholipase A2 group XIIA |
| NCBI Official Symbol: | PLA2G12A�� |
| NCBI Official Synonym Symbols: | GXII; ROSSY; PLA2G12�� |
| NCBI Protein Information: | group XIIA secretory phospholipase A2 |
| UniProt Protein Name: | Group XIIA secretory phospholipase A2 |
| UniProt Synonym Protein Names: | Phosphatidylcholine 2-acylhydrolase 12A |
| Protein Family: | Group XIIA secretory phospholipase |
| UniProt Gene Name: | PLA2G12A�� |
| UniProt Entry Name: | PG12A_HUMAN |