Human MRPS25 Recombinant Protein (RPPB4008)
- SKU:
- RPPB4008
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- MRPS25
- Synonyms:
- Mitochondrial Ribosomal Protein S25
- Mitochondrial 28S Ribosomal Protein S25
- 28S Ribosomal Protein S25 Mitochondrial
- MRP-S25
- Source:
- Escherichia Coli
- Uniprot:
- P82663
Frequently bought together:
Description
| Product Name: | Human MRPS25 Recombinant Protein |
| Product Code: | RPPB4008 |
| Size: | 20µg |
| Species: | Human |
| Target: | MRPS25 |
| Synonyms: | Mitochondrial Ribosomal Protein S25, Mitochondrial 28S Ribosomal Protein S25, 28S Ribosomal Protein S25 Mitochondrial, MRP-S25, RPMS25, S25mt. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The MRPS25 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMPMKGRF PIRRTLQYLS QGNVVFKDSV KVMTVNYNTH GELGEGARKF VFFNIPQIQY KNPWVQIMMF KNMTPSPFLR FYLDSGEQVL VDVETKSNKE IMEHIRKILG KNEETLREEE EEKKQLSHPA NFGPRKYCLR ECICEVEGQV PCPSLVPLPK EMRGKYKAAL KADAQD |
MRPS25, a 28S subunit protein, is a member of the ribosomal protein S5P family. An equivalent pseudogene is located on chromosome 4.
MRPS25 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-173) and having a molecular mass of 22.5kDa.MRPS25 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MRPS25: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 3p25 Cellular Component: mitochondrial inner membrane; mitochondrion; ribosome Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P82663 |
| NCBI GenInfo Identifier: | 13633894 |
| NCBI Gene ID: | 64432 |
| NCBI Accession: | P82663.1 |
| UniProt Secondary Accession: | P82663,Q9H7P5, B4DFJ5, B4DQG6, |
| UniProt Related Accession: | P82663 |
| Molecular Weight: | 14,953 Da |
| NCBI Full Name: | 28S ribosomal protein S25, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein S25 |
| NCBI Official Symbol: | MRPS25 |
| NCBI Official Synonym Symbols: | RPMS25; MRP-S25 |
| NCBI Protein Information: | 28S ribosomal protein S25, mitochondrial |
| UniProt Protein Name: | 28S ribosomal protein S25, mitochondrial |
| Protein Family: | 28S ribosomal protein |
| UniProt Gene Name: | MRPS25 |
| UniProt Entry Name: | RT25_HUMAN |
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