Human MDH1 Recombinant Protein (RPPB1929)
- SKU:
- RPPB1929
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- MDH1
- Synonyms:
- MDH-s
- MDHA
- MOR2
- Source:
- Escherichia Coli
- Uniprot:
- P40925
Description
| Product Name: | Human MDH1 Recombinant Protein |
| Product Code: | RPPB1929 |
| Size: | 25µg |
| Species: | Human |
| Target: | MDH1 |
| Synonyms: | MDH-s, MDHA, MOR2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MDH1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH |
| Biological Activity: | Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C. |
MDH1 catalyzes the reversible oxidation of malate to oxaloacetate, using the NAD/NADH cofactor system in the citric acid cycle. MDH1 is abundantly found in the cytoplasm and is involved in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH1 regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation.
MDH1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 342 amino acids (1-334 a.a.) and having a molecular mass of 37.4 kDa. The MDH1 is fused to an 8 amino acid His tag at C-terminus and purified by conventional chromatography.
| UniProt Protein Function: | MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010] |
| UniProt Protein Details: | Protein type:EC 1.1.1.96; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol Molecular Function:malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P40925 |
| NCBI GenInfo Identifier: | 1708967 |
| NCBI Gene ID: | 4190 |
| NCBI Accession: | P40925.4 |
| UniProt Related Accession: | P40925 |
| Molecular Weight: | ~ 36kDa |
| NCBI Full Name: | Malate dehydrogenase, cytoplasmic |
| NCBI Synonym Full Names: | malate dehydrogenase 1 |
| NCBI Official Symbol: | MDH1 |
| NCBI Official Synonym Symbols: | MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 |
| NCBI Protein Information: | malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal |
| UniProt Protein Name: | Malate dehydrogenase, cytoplasmic |
| UniProt Synonym Protein Names: | Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96) |
| Protein Family: | Malate dehydrogenase |
| UniProt Gene Name: | MDH1 |
| UniProt Entry Name: | MDHC_HUMAN |
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