Human Insulin ELISA Kit (HDES0114)
- SKU:
- HDES0114
- Size:
- 96T
- Detection Method:
- Sandwich ELISA
- Target:
- INS
- Detection Wavelength:
- 450/630nm
Description
Product Name: | Human Insulin ELISA Kit |
SKU: | HDES0114 |
Size: | 96T |
Target: | INS |
Detection Method: | Sandwich ELISA |
Result Type: | Quantitative: Sensitivity 99.3%, Specificity 99.8% |
Sample Type: | Serum |
Assay Time: | 1 h 15 min |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Tuberculosis (TB) IgG in human serum, www.vetassay-elab.com 2 Enzyme linked immunosorbent assay (ELISA) sandwich method was used to detect the content of human insulin in serum. Firstly, a monoclonal antibody is coated onto a microplate to prepare a solid-phase antibody. Then, the serum to be tested and another monoclonal antibody labeled with horseradish peroxidase are added to form a complex of coated antibody insulin labeled antibody. After color development, the absorbance value (OD value) is measured using an enzyme-linked immunosorbent assay (ELISA) reader. The concentration absorbance curve is fitted using a computer or graph to calculate the human insulin content in the serum to be tested.