Description
| Product Name: | Human IL22 Recombinant Protein |
| Product Code: | RPPB0638 |
| Size: | 5µg |
| Species: | Human |
| Target: | IL22 |
| Synonyms: | IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | IL22 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACIH HHHHH |
| Biological Activity: | The ED50 as determined by its ability to induce IL-10 secretion�using COLO 205 human colorectal adenocarcinoma cell is�? 1.2 ng/ml. |
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acids signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses.
IL22 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (34-179 a.a.) �fused to a 9 aa His Tag at C-terminus containing a total of 155 amino acids and having a molecular mass of 17.8kDa.IL22 shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Cytokine Chromosomal Location of Human Ortholog: 12q15 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-22 receptor binding; cytokine activity Biological Process: cell-cell signaling; response to glucocorticoid stimulus; acute-phase response; inflammatory response |
| UniProt Code: | Q9GZX6 |
| NCBI GenInfo Identifier: | 17366814 |
| NCBI Gene ID: | 50616 |
| NCBI Accession: | Q9GZX6.1 |
| UniProt Related Accession: | Q9GZX6 |
| Molecular Weight: | Calculated MW: 20kDaObserved MW: Refer to figures |
| NCBI Full Name: | Interleukin-22 |
| NCBI Synonym Full Names: | interleukin 22 |
| NCBI Official Symbol: | IL22�� |
| NCBI Official Synonym Symbols: | TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23�� |
| NCBI Protein Information: | interleukin-22; cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor |
| UniProt Protein Name: | Interleukin-22 |
| UniProt Synonym Protein Names: | Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL22�� |
| UniProt Entry Name: | IL22_HUMAN |
