Human GUK1 Recombinant Protein (RPPB2546)
- SKU:
- RPPB2546
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- GUK1
- Synonyms:
- GMK
- GMP kinase
- Source:
- Escherichia Coli
- Uniprot:
- Q16774
Description
| Product Name: | Human GUK1 Recombinant Protein |
| Product Code: | RPPB2546 |
| Size: | 10µg |
| Species: | Human |
| Target: | GUK1 |
| Synonyms: | GMK, GMP kinase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless clear solution. |
| Formulation: | The GUK1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA |
| Biological Activity: | Specific activity is > 100 units/mg and is defined asthe amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP perminute at pH 7.5 at 37C in coupled system with PK/LDH. |
GUK1 is part of the guanylate kinase family. GUK1 occurs as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thus takes an important part in the recycling of GMP. Through its catalytic activity, GUK1 functions in regulation of the supply of guanine nucleotides to signal transduction pathways. GUK1 overexpression is related with pituitary adenocarcinomas, implicating that GUK1 is has a role in tumorigenesis.
GUK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (1-197 a.a.)and having a total molecular mass of 23.9 kDa. GUK1 is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GUK1: Essential for recycling GMP and indirectly, cGMP. Belongs to the guanylate kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.8 Chromosomal Location of Human Ortholog: 1q32-q41 Cellular Component: cytosol Molecular Function:ATP binding; guanylate kinase activity Biological Process: ATP metabolic process; dATP metabolic process; dGDP biosynthetic process; dGMP metabolic process; drug metabolic process; GDP biosynthetic process; GDP-mannose metabolic process; GMP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleotide phosphorylation; purine nucleotide metabolic process |
| NCBI Summary: | The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| UniProt Code: | Q16774 |
| NCBI GenInfo Identifier: | 4504221 |
| NCBI Gene ID: | 2987 |
| NCBI Accession: | NP_000849.1 |
| UniProt Secondary Accession: | Q16774,B1ANH1, |
| UniProt Related Accession: | Q16774 |
| Molecular Weight: | 37.6 kDa |
| NCBI Full Name: | guanylate kinase isoform b |
| NCBI Synonym Full Names: | guanylate kinase 1 |
| NCBI Official Symbol: | GUK1 |
| NCBI Official Synonym Symbols: | GMK |
| NCBI Protein Information: | guanylate kinase |
| UniProt Protein Name: | Guanylate kinase |
| UniProt Synonym Protein Names: | GMP kinase |
| Protein Family: | Guanylate kinase |
| UniProt Gene Name: | GUK1 |
| UniProt Entry Name: | KGUA_HUMAN |
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