Human GP149 full length protein-synthetic nanodisc (HDFP260)
- SKU:
- HDFP260
- Size:
- 10μg
- Target:
- GP149
- Tag:
- C-Flag Tag
- Expression Host:
- HEK293
- Conjugate:
- Unconjugated
Description
Product Name: | Nanodisc Human GP149 Protein |
SKU: | HDFP260 |
Size: | 10μg |
Target: | GP149 |
Tag: | C-Flag Tag |
Expression Host: | HEK293 |
Conjugate: | Unconjugated |
Synonyms: | IEDA, PGR10, R35 |
Protein Families: | GPCR, Transmembrane, Druggable Genome. |
Protein Description: | Human GP149 full length protein-synthetic nanodisc |
Molecular Weight: | The human full length GP149 protein has a MW of 81kDa |
This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]
Uniprot ID: | Q86SP6 |
Formulation & Reconstitution: | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping: | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Usage: | Research use only |