Description
| Product Name: | Human GCLM Recombinant Protein |
| Product Code: | RPPB1681 |
| Size: | 20µg |
| Species: | Human |
| Target: | GCLM |
| Synonyms: | Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GCLM solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.15M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS |
Glutamate-cysteine ligase (GCLM) is the first rate limiting enzyme of glutathione synthesis. The GCLM enzyme is comprised of 2 subunits, a heavy catalytic subunit and a light regulatory subunit. GCLM deficiency is associated with some forms of hemolytic anemia.
GCLM Human Recombinant produced in E. coli is a single polypeptide chain containing 298 amino acids (1-274) and having a molecular mass of 33.3kDa.GCLM is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: cytosol; glutamate-cysteine ligase complex Molecular Function:enzyme regulator activity; glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding Biological Process: glutamate metabolic process; glutathione biosynthetic process; positive regulation of glutamate-cysteine ligase activity; regulation of blood vessel size; response to drug; response to oxidative stress; sulfur amino acid metabolic process Disease: Myocardial Infarction, Susceptibility To |
| NCBI Summary: | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| UniProt Code: | P48507 |
| NCBI GenInfo Identifier: | 1346188 |
| NCBI Gene ID: | 2730 |
| NCBI Accession: | P48507.1 |
| UniProt Secondary Accession: | P48507,Q6FHC1, Q9NPX9, Q9NU74, A8K334, D3DT45, M5A959 |
| UniProt Related Accession: | P48507 |
| Molecular Weight: | 28,135 Da |
| NCBI Full Name: | Glutamate--cysteine ligase regulatory subunit |
| NCBI Synonym Full Names: | glutamate-cysteine ligase modifier subunit |
| NCBI Official Symbol: | GCLM�� |
| NCBI Official Synonym Symbols: | GLCLR�� |
| NCBI Protein Information: | glutamate--cysteine ligase regulatory subunit |
| UniProt Protein Name: | Glutamate--cysteine ligase regulatory subunit |
| UniProt Synonym Protein Names: | GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit |
| Protein Family: | Glutamate--cysteine ligase regulatory |
| UniProt Gene Name: | GCLM�� |
| UniProt Entry Name: | GSH0_HUMAN |
