Human Free Thyroxine ELISA Kit (HDES0102)
- SKU:
- HDES0102
- Size:
- 96T
- Detection Method:
- Competition ELISA
- Target:
- FT4
- Detection Wavelength:
- 450/630nm
Description
| Product Name: | Human Free Thyroxine ELISA Kit |
| SKU: | HDES0102 |
| Size: | 96T |
| Target: | FT4 |
| Detection Method: | Competition ELISA |
| Result Type: | Quantitative: Sensitivity 98.5%, Specificity 99.8% |
| Sample Type: | Serum |
| Assay Time: | 1 h 45 min |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Competition-ELISA as the method to detect the Free Thyroxine (FT4) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with anti-T4 synthetic analogue antibody. Add samples, biotin labeled T4 synthetic analogue and HRP-streptavidin conjugate in sequence. If FT4 exist in sample will competition with biotin labeled T4 synthetic analogue, finally form a anti-T4 synthetic analogue antibody, biotin labeled T4 synthetic analogue and HRP-streptavidin conjugate complex. Free components are washed away. The substrate reagent is added to initiate the color developing reaction. The presence of T4 can be determined according to the absorbance value by using a microplate reader with 450 nm (630 nm) wavelength.
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