Description
| Product Name: | Human CRADD Recombinant Protein |
| Product Code: | RPPB3246 |
| Size: | 25µg |
| Species: | Human |
| Target: | CRADD |
| Synonyms: | RAIDD, MGC9163, CRADD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain, CASP2 and RIPK1 domain containing adaptor with death domain. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CRADD protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLMLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE |
CRADD is a 22kDa, widely-expressed cytosolic adaptor/signaling protein that induces cell apoptosis/cell death in numerous tissues. CRADD is a death domain (CARD) that recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes TNFR1A, RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins.
CRADD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (1-199) and having a molecular mass of 24.9 kDa. CRADD is fused to a 20 amino acids His-Tag at N-terminus.
| UniProt Protein Function: | CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Apoptosis Chromosomal Location of Human Ortholog: 12q21.33-q23.1 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding, bridging; protein binding; protease binding Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Disease: Mental Retardation, Autosomal Recessive 34 |
| NCBI Summary: | This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with cognitive disability. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P78560 |
| NCBI GenInfo Identifier: | 2498833 |
| NCBI Gene ID: | 8738 |
| NCBI Accession: | P78560.1 |
| UniProt Related Accession: | P78560 |
| Molecular Weight: | ~ 23kDa |
| NCBI Full Name: | Death domain-containing protein CRADD |
| NCBI Synonym Full Names: | CASP2 and RIPK1 domain containing adaptor with death domain |
| NCBI Official Symbol: | CRADD�� |
| NCBI Official Synonym Symbols: | MRT34; RAIDD�� |
| NCBI Protein Information: | death domain-containing protein CRADD |
| UniProt Protein Name: | Death domain-containing protein CRADD |
| UniProt Synonym Protein Names: | Caspase and RIP adapter with death domain; RIP-associated protein with a death domain |
| UniProt Gene Name: | CRADD�� |
| UniProt Entry Name: | CRADD_HUMAN |
