Human CA3 Recombinant Protein (RPPB1460)
- SKU:
- RPPB1460
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- CA3
- Synonyms:
- Car3
- CAIII
- Carbonic anhydrase 3
- EC 4211
- Source:
- Escherichia Coli
- Uniprot:
- P07451
Description
| Product Name: | Human CA3 Recombinant Protein |
| Product Code: | RPPB1460 |
| Size: | 20µg |
| Species: | Human |
| Target: | CA3 |
| Synonyms: | Car3, CAIII, Carbonic anhydrase 3, EC 4.2.1.1, Carbonic anhydrase III, Carbonate dehydratase III, CA-III. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The CA3 solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAKEWGYASH NGPDHWHELF PNAKGENQSP IELHTKDIRH DPSLQPWSVS YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI NNRVVRASFK |
Carbonic anhydrase III is part of a multigene family that encodes carbonic anhydrase isozymes which are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in various cell types. Carbonic anhydrase III expression is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. CA3 catalyses swift conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O = HCO3 + H+). CA3 participates in a variety of biological processes, including respiration, calcifica-tion, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. CA3 includes a zinc ion in its active site and maintains acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues.
CA3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 260 amino acids (1-260 a.a.) and having a molecular mass of 29.5 kDa. The CA3 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase Chromosomal Location of Human Ortholog: 8q21.2 Cellular Component: cytosol Molecular Function:carbonate dehydratase activity; zinc ion binding; nickel ion binding Biological Process: response to ethanol; bicarbonate transport; one-carbon compound metabolic process; response to oxidative stress |
| NCBI Summary: | Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P07451 |
| NCBI GenInfo Identifier: | 134047703 |
| NCBI Gene ID: | 761 |
| NCBI Accession: | P07451.3 |
| UniProt Secondary Accession: | P07451,O60842, B2R867, B3KUC8, |
| UniProt Related Accession: | P07451 |
| Molecular Weight: | 260 |
| NCBI Full Name: | Carbonic anhydrase 3 |
| NCBI Synonym Full Names: | carbonic anhydrase III, muscle specific |
| NCBI Official Symbol: | CA3 |
| NCBI Official Synonym Symbols: | Car3; CAIII |
| NCBI Protein Information: | carbonic anhydrase 3; CA-III; HEL-S-167mP; carbonate dehydratase III; epididymis secretory sperm binding protein Li 167mP |
| UniProt Protein Name: | Carbonic anhydrase 3 |
| UniProt Synonym Protein Names: | Carbonate dehydratase III; Carbonic anhydrase III; CA-III |
| Protein Family: | Carbonic anhydrase |
| UniProt Gene Name: | CA3 |
| UniProt Entry Name: | CAH3_HUMAN |
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