Human APRT Recombinant Protein (RPPB1406)
- SKU:
- RPPB1406
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- APRT
- Synonyms:
- EC 24273
- MGC125857
- AMP diphosphorylase
- Adenine phosphoribosyltransferase
- Source:
- Escherichia Coli
- Uniprot:
- P07741
Description
| Product Name: | Human APRT Recombinant Protein |
| Product Code: | RPPB1406 |
| Size: | 20µg |
| Species: | Human |
| Target: | APRT |
| Synonyms: | EC 2.4.2.73, MGC125857, AMP diphosphorylase, Adenine phosphoribosyltransferase, APRT, AMP, MGC125856, MGC129961, DKFZp686D13177. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The protein solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE |
APRT is part of the purine/pyrimidine phosphoribosyltransferase family. APRT enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). APRT produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in APRT causes 2,8-dihydroxyadenine urolithiasis. APRT catalyzes a salvage reaction resulting in the formation of AMP.
APRT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 180 amino acids (1-180 a.a.) and having a molecular mass of 19.6 kDa. The APRT is purified by conventional chromatography.
| UniProt Protein Function: | APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Transferase; EC 2.4.2.7 Chromosomal Location of Human Ortholog: 16q24 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:adenine binding; adenine phosphoribosyltransferase activity; AMP binding Biological Process: grooming behavior; lactation; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process Disease: Adenine Phosphoribosyltransferase Deficiency |
| NCBI Summary: | Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07741 |
| NCBI GenInfo Identifier: | 114074 |
| NCBI Gene ID: | 353 |
| NCBI Accession: | P07741.2 |
| UniProt Secondary Accession: | P07741,Q3KP55, Q68DF9, G5E9J2, |
| UniProt Related Accession: | P07741 |
| Molecular Weight: | 14,557 Da |
| NCBI Full Name: | Adenine phosphoribosyltransferase |
| NCBI Synonym Full Names: | adenine phosphoribosyltransferase |
| NCBI Official Symbol: | APRT |
| NCBI Official Synonym Symbols: | AMP; APRTD |
| NCBI Protein Information: | adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase |
| UniProt Protein Name: | Adenine phosphoribosyltransferase |
| Protein Family: | Probable adenine phosphoribosyltransferase |
| UniProt Gene Name: | APRT |
| UniProt Entry Name: | APT_HUMAN |
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