Human AES Recombinant Protein (RPPB2707)
- SKU:
- RPPB2707
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- AES
- Synonyms:
- Amino-terminal enhancer of split
- Amino enhancer of split
- Gp130-associated protein
- GAM
- Source:
- Escherichia Coli
- Uniprot:
- Q08117
Description
| Product Name: | Human AES Recombinant Protein |
| Product Code: | RPPB2707 |
| Size: | 10µg |
| Species: | Human |
| Target: | AES |
| Synonyms: | Amino-terminal enhancer of split, Amino enhancer of split, Gp130-associated protein, GAM, Protein ESP1, Protein GRG, AES, GRG, ESP1, GRG5, TLE5, AES-1, AES-2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The AES solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD |
Amino-terminal enhancer of split (AES) is a member of the groucho/TLE family of proteins. AES can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. Furthermore, AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein which is involved in neurogenesis during embryonic development. The AES protein is expressed predominately in the fetal brain, liver, lung, heart and kidney and also in the adult muscle.
AES Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (1-197 a.a.) and having a molecular mass of 24.1kDa. The AES is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleus Molecular Function:protein binding; transcription corepressor activity Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development |
| NCBI Summary: | The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q08117 |
| NCBI GenInfo Identifier: | 23503062 |
| NCBI Gene ID: | 166 |
| NCBI Accession: | Q08117.4 |
| UniProt Secondary Accession: | Q08117,Q12808, Q14CJ1, Q96TG9, Q9UDY9, B2RBL0, |
| UniProt Related Accession: | Q08117 |
| Molecular Weight: | 29,091 Da |
| NCBI Full Name: | Amino-terminal enhancer of split |
| NCBI Synonym Full Names: | amino-terminal enhancer of split |
| NCBI Official Symbol: | AES |
| NCBI Official Synonym Symbols: | GRG; ESP1; GRG5; TLE5; AES-1; AES-2 |
| NCBI Protein Information: | amino-terminal enhancer of split; gp130-associated protein GAM |
| UniProt Protein Name: | Amino-terminal enhancer of split |
| UniProt Synonym Protein Names: | Gp130-associated protein GAM; Protein ESP1; Protein GRG |
| Protein Family: | Acetyl esterase |
| UniProt Gene Name: | AES |
| UniProt Entry Name: | AES_HUMAN |
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