HRP-conjugated Goat anti-Rabbit IgG Heavy Chain (CABS063)
The HRP Conjugated Goat Anti-Rabbit IgG Heavy Chain (CAB5063) is a specialized antibody designed for research involving the detection and analysis of rabbit IgG heavy chain proteins. This antibody, conjugated with horseradish peroxidase (HRP), allows for enhanced sensitivity and signal amplification in various immunoassays such as Western blotting and ELISA.Raised in goats, this antibody exhibits high specificity and affinity towards rabbit IgG heavy chain proteins, making it a valuable tool for researchers studying antibody structure and function. Its conjugation with HRP allows for easy detection and visualization of target proteins, providing accurate and reliable results in experimental settings.
With its wide range of applications in molecular biology, immunology, and protein research, the HRP Conjugated Goat Anti-Rabbit IgG Heavy Chain (CAB5063) is an essential reagent for laboratories conducting studies on antibody biology, protein-protein interactions, and immune response mechanisms. Its versatility and reliability make it a valuable asset for scientists conducting cutting-edge research in these fields.
Product Name:
HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
SKU:
CABS063
Size:
100uL
Isotype:
IgG
Host Species:
Goat
Immunogen:
Rabbit IgG
Tested Applications:
WB
Recommended Dilution:
WB,1:1000-10000
Positive Sample:
Rabbit IgG,Mouse IgG
Conjugate:
HRP
Observed MW:
55kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 50% glycerol,pH7.3.
Western blot analysis of lysates from Rabbit and Mouse IgG, using HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain (CABS063) at 1:5000 dilution.Lysates/proteins: 100ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
