Description
Product Name: GeniePlex Human Kidney Toxicity 5-Plex
Analytes
IP-10, TIM-1, OPN, NGAL, Cystatin C
Species Reactivity:
Human
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
This GeniePlex Human Kidney Toxicity 5-Plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IP-10, TIM-1, OPN, NGAL, Cystatin C on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 5 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
| Reactivity: | Human |
| Analytes: | IP-10, TIM-1, OPN, NGAL, Cystatin C |
| 1x Ab-conjugated beads (premixed): | S4P3 - IP-10, S4P6 - TIM-1, S4P7 - OPN, S5P2 - NGAL, S5P9 - Cystatin C |
| 2x Biotin-detection Ab (premixed): | Biotin - IP-10, Biotin - TIM-1, Biotin - OPN, Biotin - NGAL, Biotin - Cystatin C |
| Buffers: | 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
| Lyophilized Standard Mix: | Lyophilized Standard Mix-Human Kidney Toxicity 5-Plex. One vial containing lyophilized recombinant IP-10, TIM-1, OPN, NGAL, Cystatin C. |
| Sample types: | Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
| Sensitivity (LODs): | Cystatin C, < 20 pg/mL; IP-10, < 3 pg/mL; NGAL, < 50 pg/mL; OPN, < 5 pg/mL; TIM-1, < 5 pg/mL. |
| LLOQs: | Cystatin C, < 40 pg/mL; IP-10, < 10 pg/mL; NGAL, < 100 pg/mL; OPN, < 10 pg/mL; TIM-1, < 10 pg/mL. |
| ULOQs: | Cystatin C, > 20,000 pg/mL; IP-10, > 2,000 pg/mL; NGAL, > 50,000 pg/mL; OPN, > 5,000 pg/mL; TIM-1, > 5,000 pg/mL. |
| Standard dose recovery: | Standard dose recovery: 70-130% |
| Intra-assay CV: | Intra-assay CV: < 10% |
| Inter-assay CV: | Inter-assay CV: < 20% |
| Cross-reactivity of analytes in the Panel: | Cross-reactivity of analytes in the Panel: Negligible |
| Sample volume: | Sample volume: 15 µL/test |
Datasheets
