The General Prostaglandin H2 (PGH2) ELISA Kit from Assay Genie is a highly sensitive and specific assay designed for the accurate detection of PGH2 levels in various sample types including serum, plasma, and tissue homogenates.Prostaglandin H2 is a key intermediate in the biosynthesis of various prostaglandins and thromboxanes, which play important roles in inflammation, platelet aggregation, and regulation of blood pressure. Abnormal levels of PGH2 have been implicated in various pathological conditions such as cardiovascular diseases, asthma, and inflammatory disorders.
This ELISA kit offers a reliable and reproducible method for quantifying PGH2 levels in biological samples, making it an essential tool for researchers studying the role of prostaglandins in health and disease. With its user-friendly protocol and high performance characteristics, the General Prostaglandin H2 (PGH2) ELISA Kit is a valuable asset for any laboratory conducting research in the field of lipid mediators and inflammatory pathways.
Product Name:
General Prostaglandin H2 (PGH2) ELISA Kit
Product Code:
UNDL00065
Size:
96 Assays
Target:
PGH2
Synonyms:
PG-H2
Detection Method:
Competition
Range:
15.6-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
