The UBA3 Polyclonal Antibody (CAB20902) is a vital tool for researchers studying UBA3, a key component of the NEDD8-activating enzyme (NAE) complex. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot and immunohistochemistry applications. By targeting the UBA3 protein, this antibody enables the detection and analysis of UBA3 expression in various cell types, making it an essential reagent for investigations in the fields of protein degradation, cell cycle regulation, and cancer biology.
UBA3 is essential for the activation of the small ubiquitin-like modifier (SUMO) modification pathway, playing a crucial role in protein stability, DNA repair, and cell signaling processes. Dysregulation of UBA3 has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections, making it a promising target for therapeutic interventions. Unraveling the functions of UBA3 is essential for understanding the molecular mechanisms underlying these diseases and developing targeted strategies for their treatment.
Product Name:
UBA3 Monoclonal Antibody
SKU:
CAB20902
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human UBA3 (NP_003959.3).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9039
Clone Number:
ARC2865
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UBA3 antibody (CAB20902) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
