The Anti-Symmetric Dimethyl Histone H3 R8 Antibody (CAB20820) is a powerful tool for researchers studying epigenetics and gene regulation. This antibody, raised in rabbits, specifically recognizes the symmetric dimethyl histone H3 R8 modification, a crucial mark associated with gene activation and transcriptional regulation. Validated for use in various applications such as Western blot and immunofluorescence, this antibody allows for the detection and analysis of the symmetric dimethyl histone H3 R8 modification in human samples. Its high reactivity and specificity make it an excellent choice for investigating the role of this epigenetic mark in processes such as chromatin remodeling, DNA repair, and cell differentiation.
The symmetric dimethyl histone H3 R8 modification is known to play a role in gene expression and is implicated in various diseases including cancer, neurological disorders, and cardiovascular diseases. Understanding the function of this histone modification is essential for developing targeted therapies and interventions that can manipulate gene expression patterns in a precise and controlled manner. Overall, the Anti-Symmetric Dimethyl Histone H3 R8 Antibody (CAB20820) offers researchers a valuable tool for unraveling the complexities of epigenetic regulation and its implications in human health and disease.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB20820) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
