The Anti-Stella Antibody (CAB20176) is a polyclonal antibody designed for research involving Stella, a critical factor in early embryonic development and pluripotency. The antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications such as immunofluorescence and immunohistochemistry.Stella, also known as Dppa3, is a marker for pluripotent stem cells and plays a key role in maintaining the undifferentiated state of these cells. It is involved in regulating gene expression and cell differentiation processes, making it a valuable target for studies in developmental biology and regenerative medicine.
The Anti-Stella Antibody enables the detection and analysis of Stella protein in different cell types, allowing researchers to investigate its function and mechanisms of action. Understanding the role of Stella in embryonic development and stem cell maintenance has implications for various fields including stem cell research, tissue engineering, and potential therapeutic strategies for regenerative medicine.
Product Name:
Stella Rabbit Polyclonal Antibody
SKU:
CAB20176
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Stella (NP_631964.1).
cytoplasm, female pronucleus, male pronucleus, nucleus
Calculated MW:
18kDa
Observed MW:
17kDa
Enables methylated histone binding activity. Involved in negative regulation of DNA demethylation; protection of DNA demethylation of female pronucleus; and regulation of genetic imprinting. Acts upstream of or within embryonic cleavage. Located in cytoplasm; female pronucleus; and male pronucleus. Is expressed in several structures, including central nervous system; early conceptus; gonad; hindgut diverticulum; and primordial germ cell. Orthologous to human DPPA3 (developmental pluripotency associated 3).
Purification Method:
Affinity purification
Gene ID:
73708
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Stella Rabbit pAb (CAB20176) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
