The Anti-Selenok Antibody (CAB20560) offered by AssayGenie is a highly specific and reliable tool for research involving Selenok, a protein involved in selenoprotein synthesis. This antibody, raised in rabbits, has been validated for use in various applications such as Western blot and immunohistochemistry, making it suitable for studies in molecular biology and biochemistry.Selenok plays a crucial role in the incorporation of selenium into selenoproteins, which are essential for antioxidant defense and thyroid hormone metabolism. Dysregulation of selenoprotein synthesis has been implicated in various human diseases, including cancer, cardiovascular disease, and neurodegenerative disorders.
By targeting Selenok with this antibody, researchers can investigate its role in selenium metabolism and its potential implications in disease pathogenesis.Overall, the Anti-Selenok Antibody (CAB20560) is a valuable tool for scientists studying selenoprotein synthesis, selenium metabolism, and the impact of selenok in health and disease. Its high specificity and sensitivity make it an ideal choice for accurate and reliable detection of Selenok in various experimental settings.
Product Name:
SELENOK Rabbit Polyclonal Antibody
SKU:
CAB20560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-94 of human SELENOK (NP_067060.2).
The protein encoded by this gene belongs to the selenoprotein K family. It is a transmembrane protein that is localized in the endoplasmic reticulum (ER), and is involved in ER-associated degradation (ERAD) of misfolded, glycosylated proteins. It also has a role in the protection of cells from ER stress-induced apoptosis. Knockout studies in mice show the importance of this gene in promoting Ca(2+) flux in immune cells and mounting effective immune response. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Pseudogenes of this locus have been identified on chromosomes 6 and 19.
Purification Method:
Affinity purification
Gene ID:
58515
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SELENOK antibody (CAB20560) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.