The SEC63 Polyclonal Antibody (CAB20554) is a valuable tool for research involving SEC63, a protein involved in protein translocation and folding in the endoplasmic reticulum. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SEC63 protein, this antibody allows for the detection and analysis of SEC63 in various cell types, making it an essential tool for studies in cell biology and protein regulation.SEC63 is a key component of the protein translocation machinery in the endoplasmic reticulum, playing a crucial role in ensuring proper protein folding and quality control.
Dysregulation of SEC63 has been linked to various diseases, including neurodegenerative disorders and metabolic syndromes. Therefore, studying the function of SEC63 is essential for understanding disease mechanisms and developing potential therapies.Overall, the SEC63 Polyclonal Antibody (CAB20554) is a highly specific and reliable tool for researchers studying protein translocation and folding processes in the endoplasmic reticulum. Its applications in Western blotting make it a versatile antibody for a wide range of research projects in cell biology and disease mechanisms.
Product Name:
SEC63 Rabbit Polyclonal Antibody
SKU:
CAB20554
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 595-720 of human SEC63 (NP_009145.1).
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.
Purification Method:
Affinity purification
Gene ID:
11231
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SEC63 antibody (CAB20554) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
