The RBL1 Polyclonal Antibody (CAB20518) is specially designed for research involving RBL1, a protein involved in cell cycle regulation and tumor suppression. This antibody, produced in rabbits, is highly effective in detecting RBL1 in human samples and is validated for use in various applications including Western blotting.RBL1, also known as Retinoblastoma-like protein 1, plays a crucial role in controlling cell growth and division by interacting with key proteins involved in cell cycle progression. Dysregulation of RBL1 has been implicated in various types of cancer, making it a promising target for cancer research and therapy development.
By using the RBL1 Polyclonal Antibody, researchers can study the expression and function of RBL1 in different cell types, shedding light on its role in cancer development and progression. Understanding the mechanisms by which RBL1 regulates cell cycle dynamics can provide valuable insights for the development of novel cancer treatments targeting this critical protein.
Product Name:
RBL1 Rabbit Polyclonal Antibody
SKU:
CAB20518
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RBL1 (NP_002886.2).
The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5933
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RBL1 Rabbit pAb (CAB20518) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
