Anti-Mouse CD24 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0290
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- M1/69
- Protein:
- CD24
- Isotype:
- IgG2b k
- Reactivity:
- Mouse
- Synonyms:
- Heat Stable Antigen
- Nectadrin
- Ly-52
- Research Area:
- Cell Biology
- Immunology
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- Comp InhiBlocking
- ELISA Indirect
- FACS
- FC
- IF
- IF Microscopy
- IHC
- In Vivo
- IP
- WB
Description
| Product Name: | Anti-Mouse CD24 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0290 |
| Size: | 1 mg, 5 mg, 25 mg, 50 mg, 100 mg |
| Clone: | M1/69 |
| Protein: | CD24 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Heat Stable Antigen, Nectadrin, Ly-52 |
| Isotype: | IgG2b k |
| Reactivity: | Mouse |
| Applications: | Comp Inhib, ELISA Indirect, FACS, FC, IF, IF Microscopy, IHC, In Vivo, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Applications: | Comp Inhib, ELISA Indirect, FACS, FC, IF, IF Microscopy, IHC, In Vivo, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52. |
| Antigen Distribution: | CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles. |
Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker1. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer1,2. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion3, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways5 as well as a crucial role in cell selection and maturation during hematopoiesis.
CD24 tends to be expressed more abundantly in progenitor cells and metabolically active cells relative to terminally differentiated cells2. For example, CD24 is expressed on B-cell progenitors and mature resting B cells, but not terminally differentiated plasma cells. Similarly, CD24 is abundantly expressed on immature T cells and activated T cells but weakly expressed on peripheral T cells. As such, CD24 is used as a marker for the differentiation of hematopoietic and neuronal cells as well as tumor stem cells.
In humans, CD24 is overexpressed in various malignancies and its downregulation reduces cell tumorgenicity6. When CD24 is expressed early during carcinogenesis and blocked with monoclonal antibodies or small interfering RNA, tumor growth in xenograft mouse models is reduced.
M1/69 was generated by fusing the spleen cells of a DA rat immunized with B10 mouse spleen cells enriched for T cells with cells from a nonsecreting mouse myeloma line (NSI)7.
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