The Anti-Monomethyl Histone H3 (K18) Antibody (CAB20680) is a crucial tool for researchers studying epigenetics and chromatin remodeling. This antibody specifically targets the monomethylated form of histone H3 at lysine 18, a post-translational modification associated with gene activation and transcriptional regulation.Raised in rabbits, this antibody is highly specific and sensitive when detecting monomethyl histone H3 (K18) in human samples. It has been validated for use in Western blot applications, allowing for accurate analysis of histone modifications in different cell types and biological contexts.Histone modifications, such as monomethylation of histone H3 at K18, play a key role in gene expression regulation and epigenetic control.
Understanding the impact of these modifications on cellular processes is essential for advancing research in areas such as cancer biology, developmental biology, and stem cell research.By using the Anti-Monomethyl Histone H3 (K18) Antibody (CAB20680), researchers can gain valuable insights into the role of histone modifications in gene regulation and potentially uncover new therapeutic targets for diseases influenced by epigenetic changes. This antibody is a valuable tool for advancing the field of epigenetics and unlocking the complexities of gene regulation.
Product Name:
MonoMethyl-Histone H3-K18 Monoclonal Antibody
SKU:
CAB20680
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic monomethylated peptide around K18 of human histone H3 (P68431).
Sequence:
APRK Q
Tested Applications:
WBIHC-PIF/ICCIPChIPELISADBCUT&Tag
Recommended Dilution:
DB,1:500 - 1:1000 WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of ChromatinCUT&Tag, 10⁵ cells /1 μg
HeLa nuclear extract, NIH/3T3 nuclear extract, C6 nuclear extract
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
16kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Clone Number:
ARC2621
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1μg MonoMethyl-Histone H3-K18 Rabbit mAb(CAB20680), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1), as shown in figure.
