The LIS1 Polyclonal Antibody (CAB3696) is an essential tool for research involving LIS1, a key protein involved in neuronal migration and development. This antibody, produced in rabbits, is highly specific to LIS1 in human samples and is validated for use in Western blotting and immunohistochemistry applications. By binding to LIS1, this antibody enables accurate detection and analysis of the protein in various biological samples, making it an ideal choice for studies in neuroscience and developmental biology.LIS1, also known as lissencephaly-1 protein, is crucial for proper brain development and neuronal function.
Mutations in the LIS1 gene have been associated with lissencephaly, a disorder characterized by abnormal brain development and intellectual disabilities. Research on LIS1 is essential for understanding the mechanisms underlying brain development and neuronal migration, with implications for neurodevelopmental disorders and neurodegenerative diseases.By using the LIS1 Polyclonal Antibody (CAB3696), researchers can delve deeper into the functions and interactions of LIS1 in various biological processes, paving the way for advancements in neuroscience and potential therapeutic strategies for neurological disorders.
Product Name:
LIS1 Rabbit Monoclonal Antibody
SKU:
CAB3696
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LIS1 (P43034).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
MDS; LIS1; LIS2; MDCR; NudF; PAFAH
Positive Sample:
U-87MG,Mouse testis,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
axon cytoplasm, cell cortex, central region of growth cone, centrosome, cytoplasmic microtubule, cytosol, extracellular exosome, glutamatergic synapse, neuron projection, nuclear envelope, perinuclear region of cytoplasm, Schaffer collateral - CA1 synapse
Calculated MW:
47kDa
Observed MW:
47kDa
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
Purification Method:
Affinity purification
Gene ID:
5048
Clone Number:
ARC2075
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using LIS1 Rabbit mAb (CAB3696) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
