TYMS Antibody (PACO50078)
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주문- SKU:
- PACO50078
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | TYMS Antibody (PACO50078) |
Antibody SKU: | PACO50078 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Thymidylate synthase protein (2-54AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate. All lanes: TYMS antibody at 2.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36, 32, 27 kDa. Observed band size: 36 kDa.. | |
Immunoprecipitating TYMS in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PACO50078 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO50078 (8µg) + Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg). | |
Immunofluorescent analysis of Hela cells using PACO50078 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
Synonyms: | Thymidylate synthase (TS) (TSase) (EC 2.1.1.45), TYMS, TS |
UniProt Protein Function: | TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer. |
UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Mitochondrial; Methyltransferase Chromosomal Location of Human Ortholog: 18p11.32 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol Molecular Function:mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding Biological Process: immortalization of host cell by virus; methylation; tetrahydrofolate metabolic process; circadian rhythm; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle |
NCBI Summary: | Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008] |
UniProt Code: | P04818 |
NCBI GenInfo Identifier: | 136611 |
NCBI Gene ID: | 7298 |
NCBI Accession: | P04818.3 |
UniProt Secondary Accession: | P04818,Q8WYK3, Q8WYK4, |
UniProt Related Accession: | P04818 |
Molecular Weight: | Calculated MW: 26kDa/31kDa/35kDaObserved MW: 36kDa |
NCBI Full Name: | Thymidylate synthase |
NCBI Synonym Full Names: | thymidylate synthetase |
NCBI Official Symbol: | TYMS |
NCBI Official Synonym Symbols: | TS; TMS; HST422 |
NCBI Protein Information: | thymidylate synthase; TSase |
UniProt Protein Name: | Thymidylate synthase |
Protein Family: | Thymidylate synthase |
UniProt Gene Name: | TYMS |
UniProt Entry Name: | TYSY_HUMAN |
Antibodies | ELISA Kits |
Anti-TYMS Antibody (CAB10441) | Human TYMS (Thymidylate synthase) ELISA Kit (HUFI08064) |
TYMS Antibody (PACO13007) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |