TBK1 Antibody (PACO46270)
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주문- SKU:
- PACO46270
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | TBK1 Antibody (PACO46270) |
Antibody SKU: | PACO46270 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Serine/threonine-protein kinase TBK1 protein (364-585AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse liver tissue, Mouse brain tissue. All lanes: TBK1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa. Observed band size: 84 kDa.. | |
Immunofluorescent analysis of Hela cells using PACO46270 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human testis tissue using PACO46270 at dilution of 1:100. |
Background: | Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein. |
Synonyms: | Serine/threonine-protein kinase TBK1 (EC 2.7.11.1) (NF-kappa-B-activating kinase) (T2K) (TANK-binding kinase 1), TBK1, NAK |
UniProt Protein Function: | TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. |
UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; IKK family Chromosomal Location of Human Ortholog: 12q14.1 Cellular Component: cytoplasm; endosome membrane; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; nucleic acid binding; phosphoprotein binding; ATP binding; protein kinase activity Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; MyD88-independent toll-like receptor signaling pathway; response to virus; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon type I production; positive regulation of interferon-beta production; toll-like receptor signaling pathway; interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of interferon-alpha production Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4 |
NCBI Summary: | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010] |
UniProt Code: | Q9UHD2 |
NCBI GenInfo Identifier: | 74761953 |
NCBI Gene ID: | 29110 |
NCBI Accession: | Q9UHD2.1 |
UniProt Secondary Accession: | Q9UHD2,Q8IYV3, Q9NUJ5, A8K4S4, |
UniProt Related Accession: | Q9UHD2 |
Molecular Weight: | 83,642 Da |
NCBI Full Name: | Serine/threonine-protein kinase TBK1 |
NCBI Synonym Full Names: | TANK-binding kinase 1 |
NCBI Official Symbol: | TBK1 |
NCBI Official Synonym Symbols: | NAK; T2K |
NCBI Protein Information: | serine/threonine-protein kinase TBK1; NF-kB-activating kinase; NF-kappa-B-activating kinase |
UniProt Protein Name: | Serine/threonine-protein kinase TBK1 |
UniProt Synonym Protein Names: | NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1 |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | TBK1 |
UniProt Entry Name: | TBK1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |