Phospho-MAP3K8 (Ser400) Antibody (PACO24115)
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주문- SKU:
- PACO24115
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-MAP3K8 (Ser400) Antibody (PACO24115) |
Antibody SKU: | PACO24115 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody (left)or the same antibody preincubated with blocking peptide (right). |
Background: | This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-α and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). |
Synonyms: | C-COT;; M3K8; MAP3K8; TPL2; |
UniProt Protein Function: | Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. |
UniProt Protein Details: | Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: cytoplasm; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation Disease: Lung Cancer |
NCBI Summary: | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
UniProt Code: | P41279 |
NCBI GenInfo Identifier: | 346644795 |
NCBI Gene ID: | 1326 |
NCBI Accession: | NP_001231063.1 |
UniProt Secondary Accession: | P41279,Q14275, Q5T855, Q9HC81, A8K2Q5, D3DRX1, |
UniProt Related Accession: | P41279 |
Molecular Weight: | 52,925 Da |
NCBI Full Name: | mitogen-activated protein kinase kinase kinase 8 |
NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 8 |
NCBI Official Symbol: | MAP3K8 |
NCBI Official Synonym Symbols: | COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT |
NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase |
UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 8 |
UniProt Synonym Protein Names: | Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2 |
UniProt Gene Name: | MAP3K8 |
UniProt Entry Name: | M3K8_HUMAN |
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