MAP3K8 Antibody (PACO28154)
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주문- SKU:
- PACO28154
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MAP3K8 Antibody (PACO28154) |
Antibody SKU: | PACO28154 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Mitogen-activated protein kinase kinase kinase 8 protein (1-300AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO28154 at dilution of 1:100. | |
Immunofluorescent analysis of Hela cells using PACO28154 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human glioma using PACO28154 at dilution of 1:100. |
Background: | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-α (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. |
Synonyms: | Mitogen-activated protein kinase kinase kinase 8 (EC 2.7.11.25) (Cancer Osaka thyroid oncogene) (Proto-oncogene c-Cot) (Serine/threonine-protein kinase cot) (Tumor progression locus 2) (TPL-2), MAP3K8, COT ESTF |
UniProt Protein Function: | Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. |
UniProt Protein Details: | Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: cytoplasm; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation Disease: Lung Cancer |
NCBI Summary: | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
UniProt Code: | P41279 |
NCBI GenInfo Identifier: | 346644795 |
NCBI Gene ID: | 1326 |
NCBI Accession: | NP_001231063.1 |
UniProt Secondary Accession: | P41279,Q14275, Q5T855, Q9HC81, A8K2Q5, D3DRX1, |
UniProt Related Accession: | P41279 |
Molecular Weight: | 52,925 Da |
NCBI Full Name: | mitogen-activated protein kinase kinase kinase 8 |
NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 8 |
NCBI Official Symbol: | MAP3K8 |
NCBI Official Synonym Symbols: | COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT |
NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase |
UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 8 |
UniProt Synonym Protein Names: | Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2 |
UniProt Gene Name: | MAP3K8 |
UniProt Entry Name: | M3K8_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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