Phospho-FOXO4 (Ser197) Antibody (PACO24469)
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주문- SKU:
- PACO24469
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-FOXO4 (Ser197) Antibody (PACO24469) |
Antibody SKU: | PACO24469 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody. | |
Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
Background: | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. |
Synonyms: | AFX; FOXO4; AFX1; Afxh; |
UniProt Protein Function: | FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described. |
UniProt Protein Details: | Protein type:Oncoprotein; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: Xq13.1 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:beta-catenin binding; DNA binding; enzyme binding; identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: cell cycle arrest; insulin receptor signaling pathway; negative regulation of angiogenesis; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; stem cell differentiation; transcription from RNA polymerase II promoter |
NCBI Summary: | This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] |
UniProt Code: | P98177 |
NCBI GenInfo Identifier: | 110825720 |
NCBI Gene ID: | 4303 |
NCBI Accession: | P98177.5 |
UniProt Secondary Accession: | P98177,O43821, Q13720, Q3KPF1, Q8TDK9, B7WPJ7, |
UniProt Related Accession: | P98177 |
Molecular Weight: | 47,941 Da |
NCBI Full Name: | Forkhead box protein O4 |
NCBI Synonym Full Names: | forkhead box O4 |
NCBI Official Symbol: | FOXO4 |
NCBI Official Synonym Symbols: | AFX; AFX1; MLLT7 |
NCBI Protein Information: | forkhead box protein O4 |
UniProt Protein Name: | Forkhead box protein O4 |
UniProt Synonym Protein Names: | Fork head domain transcription factor AFX1 |
Protein Family: | Forkhead box protein |
UniProt Gene Name: | FOXO4 |
UniProt Entry Name: | FOXO4_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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