Phospho-FOXO1/FOXO3/FOXO4 (Thr24/32) Antibody (PACO24086)
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주문- SKU:
- PACO24086
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-FOXO1/FOXO3/FOXO4 (Thr24/32) Antibody (PACO24086) |
Antibody SKU: | PACO24086 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of threonine 24/32(S-C-T(p)-W-P) derived from Human FOXO1/3/4-pan . |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from 293 cells treated with Serum using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody.The lane on the right is treated with the antigen-specific peptide. |
Background: | Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. |
Synonyms: | FKHR; FOXO1; FKHR2; AFX; MLLT7 |
UniProt Protein Function: | FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain. |
UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Oncoprotein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 13q14.1 Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus Molecular Function:beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent Disease: Rhabdomyosarcoma 2 |
NCBI Summary: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q12778 |
NCBI GenInfo Identifier: | 116241368 |
NCBI Gene ID: | 2308 |
NCBI Accession: | Q12778.2 |
UniProt Secondary Accession: | Q12778,O43523, Q5VYC7, Q6NSK6, |
UniProt Related Accession: | Q12778 |
Molecular Weight: | |
NCBI Full Name: | Forkhead box protein O1 |
NCBI Synonym Full Names: | forkhead box O1 |
NCBI Official Symbol: | FOXO1 |
NCBI Official Synonym Symbols: | FKH1; FKHR; FOXO1A |
NCBI Protein Information: | forkhead box protein O1 |
UniProt Protein Name: | Forkhead box protein O1 |
UniProt Synonym Protein Names: | Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
Protein Family: | Foxo1-corepressor |
UniProt Gene Name: | FOXO1 |
UniProt Entry Name: | FOXO1_HUMAN |
Antibodies |
Anti-FoxO1/FoxO3a/FoxO4 Antibody (CAB17978) |
Phospho-FOXO1/FOXO3/FOXO4 (T24/32) Antibody (PACO03684) |
FOXO1/FOXO3/FOXO4 Antibody (PACO03685) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |