Phospho-EIF4E (Ser209) Antibody (PACO24482)
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주문- SKU:
- PACO24482
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-EIF4E (Ser209) Antibody (PACO24482) |
Antibody SKU: | PACO24482 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody. | |
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
Background: | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. |
Synonyms: | mRNA cap-binding protein; eIF-4F 25 kDa subunit; |
UniProt Protein Function: | EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. |
UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 4q23 Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol Molecular Function:protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development Disease: Autism, Susceptibility To, 19 |
NCBI Summary: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
UniProt Code: | P06730 |
NCBI GenInfo Identifier: | 1352435 |
NCBI Gene ID: | 1977 |
NCBI Accession: | P06730.2 |
UniProt Secondary Accession: | P06730,Q96E95, B7Z6V1, D6RCQ6, |
UniProt Related Accession: | P06730 |
Molecular Weight: | 27,260 Da |
NCBI Full Name: | Eukaryotic translation initiation factor 4E |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E |
NCBI Official Symbol: | EIF4E |
NCBI Official Synonym Symbols: | CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1 |
NCBI Protein Information: | eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein |
UniProt Protein Name: | Eukaryotic translation initiation factor 4E |
UniProt Synonym Protein Names: | eIF-4F 25 kDa subunit; mRNA cap-binding protein |
Protein Family: | Eukaryotic translation initiation factor |
UniProt Gene Name: | EIF4E |
UniProt Entry Name: | IF4E_HUMAN |
Antibodies |
Anti-EIF4E Antibody (CAB0468) |
Anti-EIF4E Antibody (CAB2162) |
Anti-EIF4E Antibody (CAB6085) |
Anti-Phospho-eIF4E-S209 Antibody (CABP0747) |
Anti-EIF4E Antibody (CAB19044) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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