NEK4 Antibody (PACO56896)
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주문- SKU:
- PACO56896
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | NEK4 Antibody (PACO56896) |
Antibody SKU: | PACO56896 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Serine/threonine-protein kinase Nek4 protein (516-661AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of Hela cells with PACO56896 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage. |
Synonyms: | Serine/threonine-protein kinase Nek4 (EC 2.7.11.1) (Never in mitosis A-related kinase 4) (NimA-related protein kinase 4) (Serine/threonine-protein kinase 2) (Serine/threonine-protein kinase NRK2), NEK4, STK2 |
UniProt Protein Function: | NEK4: a protein kinase of the NEK family. Highly conserved between the mouse and human and expressed at high levels in the testis. |
UniProt Protein Details: | Protein type:Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytoplasm; cilium Molecular Function:protein serine/threonine kinase activity; manganese ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: mitosis; cell division; mitotic cell cycle; protein amino acid phosphorylation; response to DNA damage stimulus |
UniProt Code: | P51957 |
NCBI GenInfo Identifier: | 116283241 |
NCBI Gene ID: | 6787 |
NCBI Accession: | |
UniProt Related Accession: | P51957,Q05DF6 |
Molecular Weight: | 50,822 Da |
NCBI Full Name: | NEK4 protein |
NCBI Synonym Full Names: | NIMA (never in mitosis gene a)-related kinase 4 |
NCBI Official Symbol: | NEK4 |
NCBI Official Synonym Symbols: | NRK2; STK2; pp12301; MGC33171 |
NCBI Protein Information: | serine/threonine-protein kinase Nek4; OTTHUMP00000213580; OTTHUMP00000213581; OTTHUMP00000213582; serine/threonine kinase 2; nimA-related protein kinase 4; serine/threonine protein kinase-2; serine/threonine-protein kinase 2; never in mitosis A-related kinase 4; serine/threonine-protein kinase NRK2 |
UniProt Protein Name: | NEK4 protein |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | NEK4 |
UniProt Entry Name: | Q05DF6_HUMAN |