NCF1 (Ab-345) Antibody (PACO23084)
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주문- SKU:
- PACO23084
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | NCF1 (Ab-345) Antibody (PACO23084) |
Antibody SKU: | PACO23084 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
Species Reactivity: | Human |
Immunogen: | Synthesized non-phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-S(p)-P-G). |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using p47 phox (Ab-345) antibody. | |
Immunohistochemistry analysis of paraffin-embedded human brain tissue using p47 phox (Ab-345) antibody. |
Background: | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
Synonyms: | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-1; NCF-47K; NCF1 |
UniProt Protein Function: | p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease. |
UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.-.-.- Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex Molecular Function:electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell proliferation; cell redox homeostasis; cellular defense response; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; leukotriene metabolic process; negative regulation of smooth muscle contraction; protein targeting to membrane; respiratory burst; respiratory burst during defense response; response to yeast; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I |
NCBI Summary: | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
UniProt Code: | P14598 |
NCBI GenInfo Identifier: | 325511390 |
NCBI Gene ID: | 653361 |
NCBI Accession: | P14598.3 |
UniProt Secondary Accession: | P14598,O43842, Q2PP07, Q53FR5, Q9BU90, Q9BXI7, Q9BXI8 Q9UDV9, Q9UMU2, A6NEH2, A8K7S9, |
UniProt Related Accession: | P14598 |
Molecular Weight: | |
NCBI Full Name: | Neutrophil cytosol factor 1 |
NCBI Synonym Full Names: | neutrophil cytosolic factor 1 |
NCBI Official Symbol: | NCF1 |
NCBI Official Synonym Symbols: | NCF1A; NOXO2; p47phox; SH3PXD1A |
NCBI Protein Information: | neutrophil cytosol factor 1 |
UniProt Protein Name: | Neutrophil cytosol factor 1 |
UniProt Synonym Protein Names: | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox |
Protein Family: | Neutrophil cytosol factor |
UniProt Gene Name: | NCF1 |
UniProt Entry Name: | NCF1_HUMAN |
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