MDH1 Antibody (PACO43528)
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주문- SKU:
- PACO43528
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MDH1 Antibody (PACO43528) |
Antibody SKU: | PACO43528 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: MDH1 antibody at 4µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 28, 39 kDa. Observed band size: 37 kDa. | |
Immunoprecipitating MDH1 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of (1µg) instead of PACO43528 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000). Lane 2: PACO43528 (4µg) + HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (20µg). | |
Immunohistochemistry of paraffin-embedded human skin tissue using PACO43528 at dilution of 1:100. |
Background: | MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid, cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. |
Synonyms: | Malate dehydrogenase, cytoplasmic (EC 1.1.1.37) (Cytosolic malate dehydrogenase) (Diiodophenylpyruvate reductase) (EC 1.1.1.96), MDH1, MDHA |
UniProt Protein Function: | MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010] |
UniProt Protein Details: | Protein type:EC 1.1.1.96; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - glyoxylate and dicarboxylate Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol Molecular Function:malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis |
NCBI Summary: | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016] |
UniProt Code: | P40925 |
NCBI GenInfo Identifier: | 1708967 |
NCBI Gene ID: | 4190 |
NCBI Accession: | P40925.4 |
UniProt Related Accession: | P40925 |
Molecular Weight: | ~ 36kDa |
NCBI Full Name: | Malate dehydrogenase, cytoplasmic |
NCBI Synonym Full Names: | malate dehydrogenase 1 |
NCBI Official Symbol: | MDH1 |
NCBI Official Synonym Symbols: | MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 |
NCBI Protein Information: | malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal |
UniProt Protein Name: | Malate dehydrogenase, cytoplasmic |
UniProt Synonym Protein Names: | Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96) |
Protein Family: | Malate dehydrogenase |
UniProt Gene Name: | MDH1 |
UniProt Entry Name: | MDHC_HUMAN |
Antibodies | ELISA Kits |
Anti-MDH1 Antibody (CAB7563) | Human Malate dehydrogenase, cytoplasmic (MDH1) ELISA Kit |
MDH1 Antibody (PACO10429) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |