KARS Antibody (PACO31868)
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주문- SKU:
- PACO31868
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | KARS Antibody (PACO31868) |
Antibody SKU: | PACO31868 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Lysine--tRNA ligase protein (101-400AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: KARS antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 72 kDa Observed band size: 69 kDa . | |
Immunofluorescent analysis of Hela cells using PACO31868 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human colon cancer using PACO31868 at dilution of 1:100. |
Background: | Catalyzes the specific attachment of an amino acid, to its cognate tRNA in a 2 step reaction: the amino acid, (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA3(Lys), the primer for reverse transcription initiation. |
Synonyms: | Lysine--tRNA ligase (EC 2.7.7.-) (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS), KARS, KIAA0070 |
UniProt Protein Function: | KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Mitochondrial; EC 6.1.1.6; Translation; Ligase Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; mitochondrial matrix; mitochondrion Molecular Function:lysine-tRNA ligase activity; protein binding Biological Process: lysyl-tRNA aminoacylation; tRNA aminoacylation for protein translation Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89 |
NCBI Summary: | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q15046 |
NCBI GenInfo Identifier: | 20178333 |
NCBI Gene ID: | 3735 |
NCBI Accession: | Q15046.3 |
UniProt Secondary Accession: | Q15046,O14946, Q96J25, Q9HB23, A8MSK1, D3DUK4, |
UniProt Related Accession: | Q15046 |
Molecular Weight: | 71,497 Da |
NCBI Full Name: | Lysine--tRNA ligase |
NCBI Synonym Full Names: | lysyl-tRNA synthetase |
NCBI Official Symbol: | KARS |
NCBI Official Synonym Symbols: | KRS; KARS1; KARS2; CMTRIB; DFNB89 |
NCBI Protein Information: | lysine--tRNA ligase |
UniProt Protein Name: | Lysine--tRNA ligase |
UniProt Synonym Protein Names: | Lysyl-tRNA synthetase; LysRS |
UniProt Gene Name: | KARS |
UniProt Entry Name: | SYK_HUMAN |
Antibodies | ELISA Kits |
Anti-KARS Antibody (CAB13772) | Human Lysyl-tRNA synthetase (KARS) ELISA Kit |
KARS Antibody (PACO10037) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |