ILK (Ab-246) Antibody (PACO23175)
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주문- SKU:
- PACO23175
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ILK (Ab-246) Antibody (PACO23175) |
Antibody SKU: | PACO23175 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthesized non-phosphopeptide derived from human ILK around the phosphorylation site of serine 246 (I-F-S(p)-H-P). |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from rat liver cells, using ILK (Ab-246) antibody. | |
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ILK (Ab-246) antibody. |
Background: | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
Synonyms: | 59 kDa serine/threonine protein kinase; EC 2.7.11.1; ILK-1; ILK1; Integrin-linked protein kinase |
UniProt Protein Function: | ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; MLK family; ILK subfamily Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: cell junction; cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm Molecular Function:protein binding; protein kinase binding; protein serine/threonine kinase activity Biological Process: cell-matrix adhesion; integrin-mediated signaling pathway; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation |
NCBI Summary: | This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
UniProt Code: | Q13418 |
NCBI GenInfo Identifier: | 9973397 |
NCBI Gene ID: | 3611 |
NCBI Accession: | Q13418.2 |
UniProt Secondary Accession: | Q13418,P57043, Q68DZ3, B7Z1I0, B7Z418, D3DQU0, |
UniProt Related Accession: | Q13418 |
Molecular Weight: | 36,467 Da |
NCBI Full Name: | Integrin-linked protein kinase |
NCBI Synonym Full Names: | integrin linked kinase |
NCBI Official Symbol: | ILK |
NCBI Official Synonym Symbols: | P59; ILK-1; ILK-2; p59ILK; HEL-S-28 |
NCBI Protein Information: | integrin-linked protein kinase |
UniProt Protein Name: | Integrin-linked protein kinase |
UniProt Synonym Protein Names: | 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK |
Protein Family: | Integrin-linked protein kinase |
UniProt Gene Name: | ILK |
UniProt Entry Name: | ILK_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |