Human TNF beta/TNFB Recombinant Protein (RPES1684)

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SKU:
RPES1684
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human TNF beta/TNFB Recombinant Protein

Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.

Product Name:Human TNF beta/TNFB Recombinant Protein (RPES1684)
Product Code:RPES1684
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Lymphotoxin-Alpha, LT-Alpha, TNF-Beta, Tumor Necrosis Factor Ligand Superfamily Member 1, LTA, TNFB, TNFSF1
Accession:P01374
Sequence:Leu35-Leu205
Fusion tag:
Activity:Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 20-80 pg/ml.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Tumor Necrosis Factor beta is produced by our E.coli expression system and the target gene encoding Leu35-Leu205 is expressed.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:18.8 kDa
AP Mol Mass:15 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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